Schools Attended:

Employers:

Work Experience:

Princeton Brain Bio Center:

• Measured trace metals in blood and food samples using atomic absorption spectroscopy.

• Measured polyamine levels in blood using column chromatography and fluorimetry.

Rutgers University Chemistry Department:

• Created 3-dimensional graphs of scientific data using a Unix-based VAX-780 computer system.

Academic Research Performed:

1. Rutgers Psychology Department Undergraduate Research Project

   Advisor: Authur Tomie, Ph.D.

• Studied behavioral cross-tolerance between phencyclidine and SKF-10047 in the rat based on lever press response rates.

• Simultaneously measured force and dansylaziridine-labeled troponin C fluorescence in skinned rabbit muscle fibers.

• Demonstrated that the decrease in muscle force due to decreased pH is directly due to action at the calcium-specific regulatory binding sites of troponin C.

2. University of Miami Molecular & Cellular Pharmacology Department

   Advisor: James Potter, Ph.D.

• Measured single cell calcium responses in vascular endothelial cells using the fluorescent calcium indicator fura-2.

• Demonstrated caffeine-sensitive calcium release and block by ryanodine.

• Examined stimulation of extracellular calcium entry by using manganese fura-2 fluorescence quench.

• Modeled cell calcium handling in endothelial cells using averaged whole cell data as well as single cell localized imaging data.

3. University of Miami Molecular & Cellular Pharmacology Department

   Advisor: David Adams, Ph.D.

Awareness Technology:

• Automated clinical chemistry assay and immunoassay development including FDA 510(k) and proficiency survey data collection and submission.

• Provide customer support and training for assay applications using the Awareness Technology ChemWell line of automated clinical analyzers.

• Custom software implementation testing and validation for automated instruments.

Viragen:

• Process development (cell culture and liquid chromatography) for the purification of natural human interferon.

• Ran and optimized ELISAs and bioassays for human interferon and process contaminates.

• Conducted final product formulation and stability studies.

Research Posters:

   Freeman, M., Van Slooten, G. and Robbins, E., Biochemistry Assays in Uncoated Microwells.  International Congress on Microplate Technology, Laboratory Automation and Robotics, June 18-21, 2001, Basel, Switzerland.  (Winner of Best Poster Award)

   Van Slooten, G., Freeman, M. and Chen, J., Feasibility of Applying Manual Microplate ELISAs to General Purpose Automated Instruments.  AACC Annual Meeting, July 25-29, 2004, Los Angeles, CA.

   Van Slooten, G., Adaptation of Neonatal Dried Blood Spot Assays for Use on Automated Microplate Instruments.  AACC Annual Meeting, July 23-27, 2023, Anaheim, CA.

Resulting Publications:

   Parsons, B., Szczesna, D., Zhao, J., Van Slooten, G., Kerrick, W.G., Putkey, J.A., and Potter, J.D.  The Effect of pH on the Calcium Affinity of the Calcium Regulatory Sites of Skeletal and Cardiac Troponin C in Skinned Muscle Fibers.  J. Mus. Res. Cell Motil., 18:599-609, 1997.

Resulting Publications:

   Adams, D.J., Rusko, J. and Van Slooten, G.  Calcium Signaling in Vascular Endothelial Cells: Calcium Entry and Release.  Ion Flux in Pulmonary Control, ed. Weir, E.K., 1993.

   Rusko, J., Van Slooten, G. and Adams, D.J.  Caffeine Evoked, Calcium Sensitive Membrane Currents in Rabbit Aortic Endothelial Cells.  Br. J. Pharm. 115:133-141, 1995.